Journal: Journal of Clinical Microbiology

Article Title: Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs ▿

doi: 10.1128/JCM.01288-09

Figure Lengend Snippet: Summary of the automated and manual DNA extraction methods used in this study

Article Snippet: Principle MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column Open in a separate window Summary of the automated and manual DNA extraction methods used in this study The four manual DNA extraction kits used three different principles for DNA extraction (Table ).

Techniques: DNA Extraction, Lysis, Binding Assay, Purification

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs ▿

doi: 10.1128/JCM.01288-09

Figure Lengend Snippet: Real-time PCR limit of detection for DNA recovered from Brucella spp. using automated and manual DNA extraction methods

Article Snippet: Principle MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column Open in a separate window Summary of the automated and manual DNA extraction methods used in this study The four manual DNA extraction kits used three different principles for DNA extraction (Table ).

Techniques: Real-time Polymerase Chain Reaction, DNA Extraction

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs ▿

doi: 10.1128/JCM.01288-09

Figure Lengend Snippet: Comparison of recovery and purity of DNA from Brucella spp. by automated and manual extraction methods

Article Snippet: Principle MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column Open in a separate window Summary of the automated and manual DNA extraction methods used in this study The four manual DNA extraction kits used three different principles for DNA extraction (Table ).

Techniques:

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs ▿

doi: 10.1128/JCM.01288-09

Figure Lengend Snippet: Real-time PCR limit of detection by the use of DNA extracted from spiked swabs

Article Snippet: Principle MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column Open in a separate window Summary of the automated and manual DNA extraction methods used in this study The four manual DNA extraction kits used three different principles for DNA extraction (Table ).

Techniques: Real-time Polymerase Chain Reaction

Journal: Journal of Clinical Microbiology

Article Title: Evaluation of Automated and Manual Commercial DNA Extraction Methods for Recovery of Brucella DNA from Suspensions and Spiked Swabs ▿

doi: 10.1128/JCM.01288-09

Figure Lengend Snippet: Comparison of costs, processing times, sample volumes, and required materials of automated and manual DNA extraction methods

Article Snippet: Principle MagNA Pure Compact Roche 03731146001 Lysis buffer and proteinase K treatments, followed by binding of nucleic acid to magnetic glass particles, magnetic separation of nucleic acid, and elution at high temperatures MagNA Pure LC Roche 12236931001 Same as for the MagNA Pure Compact instrument IT 1-2-3 DNA sample purification kit Idaho Technology 3800 Lysis buffer and bead beating, followed by binding of DNA to filter membrane, and contaminants pass through spin column MasterPure complete DNA and RNA purification kit Epicentre MC85200 Lysis buffer and proteinase K treatments, followed by RNase A treatment, protein precipitation, and DNA precipitation QIAamp DNA blood mini kit Qiagen 51106 Proteinase K treatment, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column UltraClean microbial DNA isolation kit MoBio 12224-250 Lysis buffer and bead beating, followed by binding of DNA to silica gel membrane, and contaminants pass through spin column Open in a separate window Summary of the automated and manual DNA extraction methods used in this study The four manual DNA extraction kits used three different principles for DNA extraction (Table ).

Techniques: DNA Extraction